Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association

Garg, Mansi, Gurung, Resham L, Mansoubi, Sahar, Ahmed, Jubed O, Davé, Anoushka, Watts, Felicity Z and Bianchi, Alessandro (2014) Tpz1TPP1 SUMOylation reveals evolutionary conservation of SUMO-dependent Stn1 telomere association. EMBO Reports. ISSN 1469-3178

[img]
Preview
PDF - Published Version
Download (2MB) | Preview

Abstract

Elongation of the telomeric overhang by telomerase is counteracted by synthesis of the complementary strand by the CST complex, CTC1(Cdc13)/Stn1/Ten1. Interaction of budding yeast Stn1 with overhang-binding Cdc13 is increased by Cdc13
SUMOylation. Human and fission yeast CST instead interact with overhang-binding TPP1/POT1. We show that the fission yeast TPP1 ortholog, Tpz1, is SUMOylated. Tpz1 SUMOylation restricts telomere elongation and promotes Stn1/Ten1 telomere association,and a SUMO-Tpz1 fusion protein has increased affinity for Stn1. Our data suggest that SUMO inhibits telomerase through stimulation of Stn1/Ten1 action by Tpz1, highlighting the evolutionary conservation of the regulation of CST function by SUMOylation.

Item Type: Article
Additional Information: Keywords: CST; Stn1; telomeres; Tpz1; SUMO
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Depositing User: Gee Wheatley
Date Deposited: 11 Jul 2014 09:10
Last Modified: 06 Mar 2017 18:24
URI: http://sro.sussex.ac.uk/id/eprint/49241

View download statistics for this item

📧 Request an update
Project NameSussex Project NumberFunderFunder Ref
Analysis of regulation telomerase action & telomore capping function by the telomeric complex (Fellow: Bianchi)G0129MRC-MEDICAL RESEARCH COUNCILG0701428