Sequence analysis of in vivo defective interfering-like RNA of influenza a H1N1 pandemic virus

Saira, Kazima, Lin, Xudong, DePasse, Jay V, Halpin, Rebecca, Twaddle, Alan, Stockwell, Timothy, Angus, Brian, Cozzi-Lepri, Alessandro, Delfino, Marina, Dugan, Vivien, Dwyer, Dominic E, Freiberg, Matthew, Horban, Andrzej, Losso, Marcelo, Lynfield, Ruth, Wentworth, Deborah N, Holmes, Edward C, Davey, Richard, Wentworth, David E, Ghedin, Elodie, The INSIGHT FLU002 and FLU003 Study Groups, and Newport, Melanie (2013) Sequence analysis of in vivo defective interfering-like RNA of influenza a H1N1 pandemic virus. Journal of Virology, 87 (14). pp. 8064-8074. ISSN 0022-538X

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Abstract

Influenza virus defective interfering (DI) particles are naturally occurring noninfectious virions typically generated during in vitro serial passages in cell culture of the virus at a high multiplicity of infection. DI particles are recognized for the role they play in inhibiting viral replication and for the impact they have on the production of infectious virions. To date, influenza virus DI particles have been reported primarily as a phenomenon of cell culture and in experimentally infected embryonated chicken eggs. They have also been isolated from a respiratory infection of chickens. Using a sequencing approach, we characterize several subgenomic viral RNAs from human nasopharyngeal specimens infected with the influenza A(H1N1)pdm09 virus. The distribution of these in vivo-derived DI-like RNAs was similar to that of in vitro DIs, with the majority of the defective RNAs generated from the PB2 (segment 1) of the polymerase complex, followed by PB1 and PA. The lengths of the in vivo-derived DI-like segments also are similar to those of known in vitro DIs, and the in vivo-derived DI-like segments share internal deletions of the same segments. The presence of identical DI-like RNAs in patients linked by direct contact is compatible with transmission between them. The functional role of DI-like RNAs in natural infections remains to be established.

Item Type: Article
Additional Information: Melanie Newport is not a named author of this article but is a member of the INSIGHT FLU 002 & 003 Study Groups.
Schools and Departments: Brighton and Sussex Medical School > Global Health and Infection
Subjects: R Medicine > RC Internal medicine > RC0251 Constitutional diseases (General)
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Depositing User: pam Thompson
Date Deposited: 12 May 2014 15:19
Last Modified: 03 Aug 2017 17:03
URI: http://sro.sussex.ac.uk/id/eprint/48358
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