Improving TCSPC data acquisition from CMOS SPAD arrays

Krstajić, Nikola, Poland, Simon, Tyndall, David, Walker, Richard, Coelho, Simao, Li, David D, Richardson, Justin, Ameer-Beg, Simon and Henderson, Robert (2013) Improving TCSPC data acquisition from CMOS SPAD arrays. Proceedings of SPIE, Advanced Microscopy Techniques III, 8797. p. 879709.

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Abstract

We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second). © (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE)

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics > TK8300 Photoelectronic devices (General)
Depositing User: David Day-Uei Li
Date Deposited: 24 Jun 2013 07:26
Last Modified: 19 Sep 2013 11:29
URI: http://sro.sussex.ac.uk/id/eprint/45528
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