SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae

Durand-Dubief, Mickaël, Will, William Ryan, Petrini, Edoardo, Theodorou, Delphine, Harris, Rachael R., Crawford, Margaret R., Paszkiewicz, Konrad, Krueger, Felix, Correra, Rosa Maria, Vetter, Anna T., Miller, J. Ross, Kent, Nicholas A. and Varga-Weisz, Patrick (2012) SWI/SNF-like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae. PloS Genetics, 8 (9). e1002974. ISSN 1553-7390

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Abstract

Budding yeast centromeres are sequence-defined point centromeres and are, unlike in many other organisms, not
embedded in heterochromatin. Here we show that Fun30, a poorly understood SWI/SNF-like chromatin remodeling factor
conserved in humans, promotes point centromere function through the formation of correct chromatin architecture at
centromeres. Our determination of the genome-wide binding and nucleosome positioning properties of Fun30 shows that
this enzyme is consistently enriched over centromeres and that a majority of CENs show Fun30-dependent changes in
flanking nucleosome position and/or CEN core micrococcal nuclease accessibility. Fun30 deletion leads to defects in histone
variant Htz1 occupancy genome-wide, including at and around most centromeres. FUN30 genetically interacts with CSE4,
coding for the centromere-specific variant of histone H3, and counteracts the detrimental effect of transcription through centromeres on chromosome segregation and suppresses transcriptional noise over centromere CEN3. Previous work has shown a requirement for fission yeast and mammalian homologs of Fun30 in heterochromatin assembly. As centromeres in budding yeast are not embedded in heterochromatin, our findings indicate a direct role of Fun30 in centromere chromatin by promoting correct chromatin architecture.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Depositing User: Margaret Crawford
Date Deposited: 14 May 2013 13:14
Last Modified: 09 Mar 2017 09:15
URI: http://sro.sussex.ac.uk/id/eprint/44701

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