Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores

Osborne, Mark and Boulineau, Remi (2013) Direct object resolution by image subtraction: a new molecular ruler for nanometric measurements on complexed fluorophores. Chemical Communications, 49 (1). pp. 5559-5561. ISSN 1359-7345

[img] PDF - Accepted Version
Restricted to Registered users only

Download (802kB)

Abstract

A technique for measuring distances between two or more fluorophores spaced in the 10-100 nm range is described. We identify a linear correlation between the intensity-amplitude in the difference-image of single molecules undergoing fluorescence fluctuations and their separation. The transform is used to map distances between coupled fluorophores.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Subjects: Q Science > QD Chemistry > QD0071 Analytical chemistry
Q Science > QD Chemistry > QD0450 Physical and theoretical chemistry
Depositing User: Mark Osborne
Date Deposited: 13 May 2013 08:34
Last Modified: 11 Sep 2017 17:43
URI: http://sro.sussex.ac.uk/id/eprint/44688

View download statistics for this item

📧 Request an update