The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections

Panaretou, Barry, Sinclair, Kirsty, Prodromou, Chrisostomos, Johal, Jasvinder, Pearl, Laurence and Piper, Peter W (1999) The Hsp90 of Candida albicans can confer Hsp90 functions in Saccharomyces cerevisiae: a potential model for the processes that generate immunogenic fragments of this molecular chaperone in C. albicans infections. Microbiology, 145 (12). pp. 3455-3463. ISSN 1350-0872

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Abstract

During infections with a number of important eukaryotic pathogens the Hsp90 molecular chaperone of the pathogen is recognized as an immunodominant antigen by the host immune system. Yeast molecular genetics should allow study of the extent of sequence variation within conserved immunodominant epitopes on pathogen Hsp90s that is compatible with essential Hsp90 functions, as well as the processes that generate antigenic subfragments of these Hsp90s. The Hsp90 of the fungal pathogen Candida albicans was shown in this study to provide both essential and nonessential (pheromone signalling and mammalian steroid receptor activation) Hsp90 functions in Saccharomyces cerevisiae cells. Much of the C. albicans Hsp90 expressed in respiratory S. cerevisiae cells was shown to undergo a partial degradation in vivo, a degradation that closely resembles that of the native Hsp82 (one isoform of the homologous Hsp90) in S. cerevisiae. Allowing for the differences in the length of the charged linker region between the N- and C-terminal domains of C. albicans Hsp90 and S. cerevisiae Hsp82, these two proteins expressed in S. cerevisiae appear to give the same major degradation products. These Hsp90 fragments are similar to the products of incomplete Hsp90 degradation found in C. albicans cultures.

Item Type: Article
Keywords: Antigens, Fungal/immunology Blotting, Western Candida albicans/*genetics/metabolism Candidiasis/immunology/metabolism HSP90 Heat-Shock Proteins/*genetics/immunology/*metabolism Heat-Shock Proteins/metabolism Heat-Shock Response Peptide Fragments/immunology Pheromones/metabolism Receptors, Glucocorticoid/metabolism Saccharomyces cerevisiae/*genetics/growth & development/*metabolism Saccharomyces cerevisiae Proteins Signal Transduction
Schools and Departments: School of Life Sciences > Biochemistry
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry > QD0415 Biochemistry
Q Science > QH Natural history > QH0301 Biology > QH0426 Genetics
Depositing User: Chrisostomos Prodromou
Date Deposited: 25 Feb 2015 07:25
Last Modified: 25 Feb 2015 07:25
URI: http://sro.sussex.ac.uk/id/eprint/44350
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