University of Sussex
Browse
1-s2.0-S0896627312008185-main.pdf (1.71 MB)

A preferentially segregated recycling vesicle pool of limited size supports neurotransmission in native central synapses

Download (1.71 MB)
journal contribution
posted on 2023-06-08, 14:10 authored by Vincenzo Marra, Jemima J Burden, Julian R Thorpe, Ikuko T Smith, Spencer L Smith, Michael Häusser, Tiago Branco, Kevin StarasKevin Staras
At small central synapses, efficient turnover of vesicles is crucial for stimulus-driven transmission, but how the structure of this recycling pool relates to its functional role remains unclear. Here we characterize the organizational principles of functional vesicles at native hippocampal synapses with nanoscale resolution using fluorescent dye labeling and electron microscopy. We show that the recycling pool broadly scales with the magnitude of the total vesicle pool, but its average size is small (~45 vesicles), highly variable, and regulated by CDK5/calcineurin activity. Spatial analysis demonstrates that recycling vesicles are preferentially arranged near the active zone and this segregation is abolished by actin stabilization, slowing the rate of activity-driven exocytosis. Our approach reveals a similarly biased recycling pool distribution at synapses in visual cortex activated by sensory stimulation in vivo. We suggest that in small native central synapses, efficient release of a limited pool of vesicles relies on their favored spatial positioning within the terminal

History

Publication status

  • Published

File Version

  • Published version

Journal

Neuron

ISSN

0896-6273

Publisher

Elsevier

Issue

3

Volume

76

Page range

579-589

Department affiliated with

  • Neuroscience Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Legacy Posted Date

2013-01-02

First Open Access (FOA) Date

2019-03-27

First Compliant Deposit (FCD) Date

2019-03-27

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC