Multi-electrode array recording and data analysis methods for molluscan central nervous systems

Passaro, Peter A (2012) Multi-electrode array recording and data analysis methods for molluscan central nervous systems. Doctoral thesis (PhD), University of Sussex.

[img]
Preview
PDF - Published Version
Download (8MB) | Preview

Abstract

In this work the use of the central nervous system (CNS) of the aquatic
snail Lymnaea stagnalis on planar multi-electrode arrays (MEAs) was
developed and analysis methods for the data generated were created.
A variety of different combinations of configurations of tissue from the
Lymnaea CNS were explored to determine the signal characteristics
that could be recorded by sixty channel MEAs. In particular, the
suitability of the semi-intact system consisting of the lips, oesophagus,
CNS, and associated nerve connectives was developed for use on
the planar MEA. The recording target area of the dorsal surface of
the buccal ganglia was selected as being the most promising for study
and recordings of its component cells during fictive feeding behaviour
stimulated by sucrose were made. The data produced by this type of
experimentation is very high volume and so its analysis required the
development of a custom set of software tools. The goal of this tool
set is to find the signal from individual neurons in the data streams of
the electrodes of a planar MEA, to estimate their position, and then
to predict their causal connectivity. To produce such an analysis techniques
for noise filtration, neural spike detection, and group detection
of bursts of spikes were created to pre-process electrode data streams.
The Kohonen self-organising map (SOM) algorithm was adapted for
the purpose of separating detected spikes into data streams representing
the spike output of individual cells found in the target system. A
significant addition to SOM algorithm was developed by the concurrent
use of triangulation methods based on current source density
analysis to predict the position of individual cells based on their spike
output on more than one electrode. The likely functional connectivity
of individual neurons identified by the SOM technique were analysed
through the use of a statistical causality method known as Granger
causality/causal connectivity. This technique was used to produce a
map of the likely connectivity between neural sources.

Item Type: Thesis (Doctoral)
Schools and Departments: School of Engineering and Informatics > Informatics
Subjects: Q Science > QL Zoology > QL0360 Invertebrates > QL0403 Mollusca
Depositing User: Library Cataloguing
Date Deposited: 23 Jan 2013 07:51
Last Modified: 08 Sep 2015 13:55
URI: http://sro.sussex.ac.uk/id/eprint/43341

View download statistics for this item

📧 Request an update