Insulin sensitivity (Si) assessment in lean and overweight subjects using two different protocols and updated software

Borai, Anwar, Livingstone, Callum, Shafi, Shahida, Zarif, Hawzen and Ferns, Gordon (2010) Insulin sensitivity (Si) assessment in lean and overweight subjects using two different protocols and updated software. Scandinavian journal of clinical and laboratory investigation, 70 (2). pp. 98-103. ISSN 1502-7686

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Abstract

BACKGROUND

The standard frequently-sampled intravenous glucose tolerance test (FSIVGTT) is an alternative procedure to the clamp technique for estimating the insulin sensitivity (Si) parameter. The goal of this study was to compare Si in lean and overweight individuals in addition to assessing intra-individual reproducibility using two different protocols and updated software.

METHODS

FSIVGTT was carried out in 14 lean (BMI <or= 25 kg/m(2)) and 14 overweight (BMI>25 kg/m(2)) subjects using two different protocols; full (29 samples) and short (12 samples). For reproducibility assessment four normal subjects (triplicate on three and twice on one) were recruited to undergo the same procedure at 1-week intervals. Data analysis was performed using COMAL and Minmod Millennium software.

RESULTS

Mean Si (10(-4)min(-1)[pmol/l](-1)) values were significantly different between lean and overweight subjects (p < 0.001) but not between the two protocols using both software packages. For the full and short protocols, Si values were more closely related in lean versus overweight subjects using either COMAL (r = 0.98, p < 0.001), (r = 0.89, p < 0.001) or Minmod Millennium (r = 0.99, p < 0.001), (r = 0.85, p < 0.001) software respectively. The intra-individual reproducibility (%CV) of Si (COMAL) in full versus short protocol was 18.3 +/- 11.1% and 13.7 +/- 1.9% respectively. Reproducibility values for Si (Minmod Millenium) in full versus short protocols were 14.3 +/- 3.8 and 14.9 +/- 1.9% respectively.

CONCLUSIONS

Si can be assessed accurately by a short protocol FSIVGTT in normal individuals. The short protocol may give less acceptable results for insulin sensitivity in individuals who have normal glucose tolerance but high BMI.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Division of Medical Education
Subjects: R Medicine > R Medicine (General) > R735 Medical education
Depositing User: Gordon Ferns
Date Deposited: 19 Nov 2012 12:00
Last Modified: 19 Nov 2012 12:00
URI: http://sro.sussex.ac.uk/id/eprint/42668
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