Serum insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation status in subjects with and without ischaemic heart disease

Borai, Anwar, Livingstone, Callum, Ghayour-Mobarhan, Majid, Abuosa, Ahmed, Shafi, Shahidi, Mehta, Shweta, Heidari, Alireza, Emadzadeh, Ali, Wark, Gwen and Ferns, Gordon (2010) Serum insulin-like growth factor binding protein-1 (IGFBP-1) phosphorylation status in subjects with and without ischaemic heart disease. Atherosclerosis, 208 (2). pp. 593-598. ISSN 0021-9150

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Abstract

Background: Insulin-like growth factor binding protein-1 (IGFBP-1) modulates the activity of IGF-I. It exists in serum as phosphorylated and less phosphorylated forms. We wished to measure serum levels of both these forms of IGFBP-1, using a novel assay, in subjects with, or without ischaemic heart disease (IHD).

Methods: We measured serum concentrations of the phosphorylated and less phosphorylated forms of IGFBP-1 in 75 subjects (36 with and 39 without IHD). Two immunoassays were used, one which detects non-, and less-phosphorylated forms (LpIGFBP-1), and another which specifically detects the serine phosphorylated form of IGFBP-1 (pIGFBP-1).

Results: LpIGFBP-1 concentrations were significantly higher in subjects without IHD than in those with IHD (5.3 +/- 0.5 mu g/L vs. 2.7 +/- 0.4 mu g/L, p < 0.001). pIGFBP-1 levels were also significantly higher in subjects without IHD compared to those with IHD (33.3 +/- 2.0 mu g/L vs. 25.3 +/- 2.2 mu g/L, p < 0.01). The correlation between LpIGFBP-1 and pIGFBP-1 for all subjects was (r = 0.71, p < 0.001). This association was stronger in subjects without IHD (r = 0.76, p < 0.001) than for those with IHD (r = 0.60, p < 0.001). A significant negative association was observed between IGF-I and the ratio between the two forms (r = -0.45, p < 0.0001). Receiver-Operating Characteristic (ROC) curve showed the highest area under the curve for LpIGFBP-1 (0.75) [95% CI: 0.63-0.86] and optimum cut-off value of 2.83 mu g/L with 75% sensitivity and 74% specificity.

Conclusions: We propose that low serum concentrations of IGFBP-1 forms could be a marker of coronary risk, and the LpIGFBP-1: pIGFBP-1 ratio may be an index of biologically active IGF-I

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Division of Medical Education
Subjects: R Medicine > RB Pathology > RB037 Clinical pathology. Laboratory technique
Depositing User: Gordon Ferns
Date Deposited: 12 Nov 2012 08:57
Last Modified: 12 Nov 2012 08:57
URI: http://sro.sussex.ac.uk/id/eprint/42091
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