Online chromatic and scale-space microvessel-tracing analysis for transmitted light optical images

Reyes Aldasoro, Constantino Carlos, Björndahl, Meit A, Akerman, Simon, Ibrahim, Jamila, Griffiths, Michael K and Tozer, Gillian M (2012) Online chromatic and scale-space microvessel-tracing analysis for transmitted light optical images. Microvascular Research, 84 (3). pp. 330-339. ISSN 1095-9319

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Abstract

Limited contrast in transmitted light optical images from intravital microscopy is problematic for analysing tumour vascular morphology. Moreover, in some cases, changes in vasculature are visible to a human observer but are not easy to quantify. In this paper two online algorithms are presented: scale‐space vessel tracing and chromatic decomposition for analysis of the vasculature of SW1222 human colorectal carcinoma xenografts growing in dorsal skin-fold “window” chambers in mice. Transmitted light optical images of tumours were obtained from mice treated with the tumour vascular disrupting agent, combretastatin-A-4-phosphate (CA4P), or saline. The tracing algorithm was validated against hand-traced vessels with accurate results. The measurements extracted with the algorithms confirmed the known effects of CA4P on tumour vascular topology. Furthermore, changes in the chromaticity suggest a deoxygenation of the blood with a recovery to initial levels in CA4P-treated tumours relative to the controls. The algorithms can be freely applied to other studies through the CAIMAN website (CAncer IMage ANalysis: http://www.caiman.org.uk).

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Subjects: R Medicine > R Medicine (General) > R858 Computer applications to medicine. Medical informatics
T Technology > TK Electrical engineering. Electronics Nuclear engineering
Depositing User: Constantino Reyes Aldasoro
Date Deposited: 01 Nov 2012 12:52
Last Modified: 01 Nov 2012 12:52
URI: http://sro.sussex.ac.uk/id/eprint/41728
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