T cell response to the cytomegalovirus major capsid protein (UL86) is dominated by helper cells with a large polyfunctional component and diverse epitope recognition

Fuhrmann, S, Streitz, M, Reinke, P, Volk, H D and Kern, F (2008) T cell response to the cytomegalovirus major capsid protein (UL86) is dominated by helper cells with a large polyfunctional component and diverse epitope recognition. Journal of Infectious Diseases, 197 (10). pp. 1455-1458. ISSN 0022-1899

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Abstract

T cells are crucial in controlling cytomegalovirus (CMV) infection. The CMV major capsid protein (UL86) is frequently recognized by these cells, but the nature of this response has not been explored in detail. In this study, healthy CMV-exposed individuals were examined, and ex vivo peptide stimulation of peripheral blood mononuclear cells and flow-cytometry were used to obtain data, including response prevalence, magnitude, functional profiles, and recognized epitopes. Of 24 subjects, 19 (79%) had a UL86-specific CD4 T cell response rate between 0.03% and 1.4%. This group of individuals exhibited a similar percentage of polyfunctional T cells in their UL86-specific and pp65-specific responses. A total of 8 CD4 T cell epitopes were identified. In contrast, CD8 T cell responses to UL86 were rare and small. UL86 is of interest for monitoring the response to CMV.

Item Type: Article
Keywords: Capsid Proteins/*immunology Cells, Cultured Epitopes, T-Lymphocyte/*immunology Flow Cytometry Humans Leukocytes, Mononuclear/immunology Lymphocyte Subsets/immunology T-Lymphocytes, Helper-Inducer/*immunology
Schools and Departments: Brighton and Sussex Medical School > Clinical and Experimental Medicine
Subjects: Q Science > QR Microbiology > QR0180 Immunology
Depositing User: Florian Kern
Date Deposited: 26 Oct 2012 13:57
Last Modified: 26 Jun 2013 09:04
URI: http://sro.sussex.ac.uk/id/eprint/41282
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