CMV-specific central memory T cells reside in bone marrow

Letsch, A, Knoedler, M, Na, I K, Kern, F, Asemissen, A M, Keilholz, U, Loesch, M, Thiel, E, Volk, H D and Scheibenbogen, C (2007) CMV-specific central memory T cells reside in bone marrow. European Journal of Immunology, 37 (11). pp. 3063-3068. ISSN 0014-2980

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Abstract

CMV-specific CD8(+) T cell responses in peripheral blood (PB) are characterized by a preponderance of effector and effector memory T cells. CMV-specific central memory T cells (T(CM)), which are considered crucial in maintaining long-term immunity, are rarely detectable in PB. In this study we have analyzed differentiation and function of CMV pp65-specific CD8(+) T cells in paired samples of human PB and BM using intracellular cytokine and tetramer staining. Overall frequencies of CMV pp65-specific T cells were similar in PB compared to BM; however, CMV-specific CD45RA(-)CCR7(+) T(CM) were almost exclusively detectable in BM, which was not related to a general accumulation of T(CM) in BM. In vitro, CMV-specific T cells could be more efficiently expanded from BM (median 128-fold, n=6) than from PB (median 72-fold, p=0.01). Taken together, these data show that the BM is a compartment harboring CMV-specific T(CM) and underline the concept of the BM as a secondary immune organ. CMV specific BM-derived T(CM) might be a valuable source for generating T cells for adoptive transfer.

Item Type: Article
Keywords: Aged Aged, 80 and over Bone Marrow Cells/*immunology CD8-Positive T-Lymphocytes/cytology/*immunology Cell Differentiation/immunology Cytomegalovirus/immunology Cytomegalovirus Infections/*immunology Female Flow Cytometry Humans *Immunologic Memory Male Middle Aged Phosphoproteins/immunology T-Lymphocyte Subsets/cytology/*immunology Viral Matrix Proteins/immunology
Schools and Departments: Brighton and Sussex Medical School > Clinical Medicine
Subjects: Q Science > QR Microbiology > QR0180 Immunology
Depositing User: Florian Kern
Date Deposited: 29 Oct 2012 12:09
Last Modified: 29 Oct 2012 12:09
URI: http://sro.sussex.ac.uk/id/eprint/41275
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