Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes

Ay, Bernhard, Streitz, Mathias, Boisguerin, Prisca, Schlosser, Andreas, Mahrenholz, Carsten C., Schuck, Sebastian D., Kern, Florian and Volkmer, Rudolf (2007) Sorting and pooling strategy: a novel tool to map a virus proteome for CD8 T-cell epitopes. Biopolymers, 88 (1). pp. 64-75. ISSN 0006-3525 (Print) 0006-3525 (Linking)

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Abstract

Human cytomegalovirus (CMV) is a major cause of morbidity in immunocompromised individuals. However, no efficient vaccine has been developed to date. Identification of T-cell target proteins and epitopes is crucial not only for developing a successful immunization strategy, but also for new approaches using adoptive transfer of antigen-specific T-cells. The CMV genome has more than 200 open reading frames potentially coding for as many proteins. Here, we describe a robust, fast, and simple SPOT synthesis strategy, which allowed us to micro-synthesize every possible CD8 T-cell epitope in the entire potential CMV proteome. So far, 9069 of these peptides have been tested in an ex vivo T-cell stimulation assay. As well as confirming a number of previously known epitopes, we identified several new ones.

Item Type: Article
Additional Information: Ay, Bernhard Streitz, Mathias Boisguerin, Prisca Schlosser, Andreas Mahrenholz, Carsten C Schuck, Sebastian D Kern, Florian Volkmer, Rudolf Biopolymers. 2007;88(1):64-75.
Keywords: Amino Acid Sequence Biopolymers/chemistry CD8-Positive T-Lymphocytes/*immunology Cytomegalovirus/*genetics/*immunology Epitope Mapping/*methods Epitopes/*chemistry Flow Cytometry Genome, Viral Humans Oligopeptides/chemistry Peptide Mapping Proteome Proteomics/*methods
Schools and Departments: Brighton and Sussex Medical School > Clinical Medicine
Subjects: Q Science > QD Chemistry > QD0241 Organic chemistry
Depositing User: Florian Kern
Date Deposited: 18 Oct 2012 09:52
Last Modified: 18 Oct 2012 11:22
URI: http://sro.sussex.ac.uk/id/eprint/41272
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