Apolipoprotein E (apoE) isoforms differentially induce nitric oxide production in endothelial cells

Sacre, Sandra M, Stannard, Anita K and Owen, James S (2003) Apolipoprotein E (apoE) isoforms differentially induce nitric oxide production in endothelial cells. FEBS Letters, 540 (1-3). pp. 181-187. ISSN 0014-5793

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Abstract

Although apolipoprotein E3 (apoE3) is atheroprotective, two common isoforms, apoE2 and apoE4, produce recessive and dominant hyperlipidaemias, respectively. Using a fluorescent assay, we report herein that apoE3 particles secreted from recombinant cells stimulate more nitric oxide release in cultured human EA.hy926 endothelial cells than apoE2 or apoE4 (141% more than controls vs. 61 or 11%). Phosphatidylinositol (PI) 3-kinase inhibitors suppressed the apoE effect, while apoE receptor 2 (apoER2) was tyrosine phosphorylated. We conclude that apoE stimulates endothelial nitric oxide release in an isoform-dependent manner, and propose that tyrosine phosphorylation of apoER2 initiates PI3-kinase signalling and activation of nitric oxide synthase.

Item Type: Article
Schools and Departments: Brighton and Sussex Medical School > Clinical and Laboratory Investigation
Subjects: Q Science
R Medicine
Depositing User: Sandra Sacre
Date Deposited: 01 Nov 2012 12:02
Last Modified: 01 Nov 2012 12:02
URI: http://sro.sussex.ac.uk/id/eprint/41164
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