Glutathione half-cell reduction potential and α-tocopherol as viability markers during the prolonged storage of Suaeda maritima seeds

Seal, Charlotte E, Zammit, Rosa, Scott, Peter, Flowers, Timothy J and Kranner, Ilse (2010) Glutathione half-cell reduction potential and α-tocopherol as viability markers during the prolonged storage of Suaeda maritima seeds. Seed Science Research, 20 (1). pp. 47-53. ISSN 0960-2585

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Abstract

Antioxidants protect seeds from oxidative damage during storage, supporting the maintenance of seed viability and the ability to germinate post-storage. No data on antioxidants during long-term storage are available for the seeds of the halophyte Suaeda maritima. Therefore, changes in lipid-soluble antioxidants in the tocopherol family (α-, γ-, δ-tocopherol), were investigated in seeds stored for up to 16 years at 4°C at a seed moisture content of 10–13%, as well as changes in the water-soluble antioxidant glutathione (GSH) and electrolyte leakage. Seed oil content was also measured and determined to be 22%. During the first 3 years of storage, seed viability remained high and the concentration of total tocopherol was stable. Thereafter, both seed viability and α-tocopherol concentration rapidly decreased and electrolyte leakage increased, while γ-tocopherol and δ-tocopherol concentrations did not correlate with seed viability. Although the concentrations of neither GSH nor glutathione disulphide (GSSG) alone were correlated with seed viability, the glutathione half-cell reduction potential (EGSSG/2GSH) was strongly correlated with viability throughout storage and increased before the onset of viability loss. Hence, in this species EGSSG/2GSH appeared to be an ‘early warning’ system preceding viability loss while α-tocopherol concentration changed concomitantly with viability.

Item Type: Article
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Subjects: Q Science > QK Botany > QK0710 Plant physiology
Depositing User: Catrina Hey
Date Deposited: 14 May 2012 14:03
Last Modified: 30 Nov 2012 17:13
URI: http://sro.sussex.ac.uk/id/eprint/39196
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