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The use of freeze-substitution in the preparation of plant tissue for ion localization studies
journal contribution
posted on 2023-06-08, 11:33 authored by Diana M R Harvey, J L Hall, Tim FlowersA method, utilizing freeze substitution, is described for the preparation of plant tissue for analytical electron microscopy. The fine structure of the cytoplasm was adequately preserved after freezing leaf tissue in 2-methylbutane at -170°C. Furthermore, following substitution in ether, losses of sodium and potassium from the tissue were less than 4% of the original ion content and the loss of chloride was less than 1%. The merits of the procedure as a means of tissue preparation for ion localization studies are discussed.
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Publication status
- Published
Journal
Journal of MicroscopyISSN
0022-2720Publisher
Blackwell PublishingIssue
2Volume
107Page range
189-198Department affiliated with
- Biology and Environmental Science Publications
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- No
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- Yes
Legacy Posted Date
2012-05-15Usage metrics
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