The use of freeze-substitution in the preparation of plant tissue for ion localization studies

Harvey, Diana M R, Hall, J L and Flowers, T J (1976) The use of freeze-substitution in the preparation of plant tissue for ion localization studies. Journal of Microscopy, 107 (2). pp. 189-198. ISSN 0022-2720

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Abstract

A method, utilizing freeze substitution, is described for the preparation of plant tissue for analytical electron microscopy. The fine structure of the cytoplasm was adequately preserved after freezing leaf tissue in 2-methylbutane at −170°C. Furthermore, following substitution in ether, losses of sodium and potassium from the tissue were less than 4% of the original ion content and the loss of chloride was less than 1%. The merits of the procedure as a means of tissue preparation for ion localization studies are discussed.

Item Type: Article
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Subjects: Q Science > QK Botany > QK0710 Plant physiology
Depositing User: Catrina Hey
Date Deposited: 15 May 2012 11:08
Last Modified: 30 Nov 2012 17:13
URI: http://sro.sussex.ac.uk/id/eprint/39168
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