Determination of the sodium, potassium and chloride ion concentrations in the chloroplasts of the halophyteSuaeda maritima by non-aqueous cell fractionation

Harvey, Diana M R and Flowers, T J (1978) Determination of the sodium, potassium and chloride ion concentrations in the chloroplasts of the halophyteSuaeda maritima by non-aqueous cell fractionation. Protoplasma, 97 (4). pp. 337-349. ISSN 0033-183X

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Abstract

Leaf material from the halophyteSuaeda maritima L. Dum. grown under both saline and non-saline conditions was fractionated under non-aqueous conditions in order to determine the ion content of various subcellular compartments. Fractions containing cell walls, nuclei and chloroplasts were successfully prepared and contents of DNA, chlorophyll, protein and Na+, K+, and Cl– determined. The cell wall fraction was not apparently heavily contaminated by the other fractions and had a low ion content although the nuclear fraction was contaminated by other organelles. The ion contents of chloroplasts were determined and the results discussed in relation to earlier microscopical data.

Item Type: Article
Additional Information: Times Cited: 20 Harvey, dmr flowers, tj
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Subjects: Q Science
Depositing User: Catrina Hey
Date Deposited: 14 May 2012 15:41
Last Modified: 30 Nov 2012 17:13
URI: http://sro.sussex.ac.uk/id/eprint/39163
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