Salt tolerance in the halophyte Suaeda maritima. Further properties of the enzyme malate dehydrogenase

Flowers, T J, Hall, J L and Ward, Margaret E (1976) Salt tolerance in the halophyte Suaeda maritima. Further properties of the enzyme malate dehydrogenase. Phytochemistry, 15 (8). pp. 1231-1234. ISSN 0031-9422

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Abstract

Malate dehydrogenase activity in supernatant fractions prepared from the halophyte Suaeda maritima was modified by added NACl with an optimal concentration for activation of about 50 mM. At this ionic strength of 0.05 the chlorides of sodium, potassium, ammonium, rubidium, calcium and magnesium all produced a similar degree of stimulation, while the nitrates of potassium and sodium were somewhat less effective. A similar result was obtained whether the plants were grown in the presence or absence of NACl. Furthermore, malate dehydrogenase activity in preparations from the glycophyte Pisum sativum behaved in a similar manner. The enzyme activity from both Suaeda and Pisum was separable into two fractions (I and II) by gel filtration on Sephadex G200. The MW of fraction II from Suaeda was estimated to be 165000 and that from Pisum approximately 282000: fraction I from both species eluted at the void volume of Sephadex G200. Storage of lyophilised supernatant resulted in the loss of enzyme activity from fraction I and a decrease in the overall stimulation by NaCl. Treatment of the lyophilised enzyme with NACl at a concentration of 100 mM also resulted in the loss of enzyme activity from fraction I.

Item Type: Article
Additional Information: Times Cited: 16 Flowers, tj hall, jl ward, me
Schools and Departments: School of Life Sciences > Evolution, Behaviour and Environment
Subjects: Q Science
Depositing User: Catrina Hey
Date Deposited: 16 May 2012 11:19
Last Modified: 30 Nov 2012 17:12
URI: http://sro.sussex.ac.uk/id/eprint/39095
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