Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

Li, David Day-Uei and Ameer-Beg, Simon M, et al (2012) Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays. Sensors, 12 (5). pp. 5650-5669. ISSN 1424-8220

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Abstract

We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 μm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems.
In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Informatics
Subjects: T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics > TK7885 Computer engineering. Computer hardware
T Technology > TK Electrical engineering. Electronics Nuclear engineering > TK7800 Electronics > TK8300 Photoelectronic devices (General)
Depositing User: David Day-Uei Li
Date Deposited: 21 May 2012 15:16
Last Modified: 12 Mar 2017 22:04
URI: http://sro.sussex.ac.uk/id/eprint/38889

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