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Time-Domain Fluorescence Lifetime Imaging Techniques Suitable for Solid-State Imaging Sensor Arrays

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journal contribution
posted on 2023-06-08, 11:27 authored by David Day-Uei Li, Simon M, et al Ameer-Beg
We have successfully demonstrated video-rate CMOS single-photon avalanche diode (SPAD)-based cameras for fluorescence lifetime imaging microscopy (FLIM) by applying innovative FLIM algorithms. We also review and compare several time-domain techniques and solid-state FLIM systems, and adapt the proposed algorithms for massive CMOS SPAD-based arrays and hardware implementations. The theoretical error equations are derived and their performances are demonstrated on the data obtained from 0.13 µm CMOS SPAD arrays and the multiple-decay data obtained from scanning PMT systems. In vivo two photon fluorescence lifetime imaging data of FITC-albumin labeled vasculature of a P22 rat carcinosarcoma (BD9 rat window chamber) are used to test how different algorithms perform on bi-decay data. The proposed techniques are capable of producing lifetime images with enough contrast.

History

Publication status

  • Published

File Version

  • Published version

Journal

Sensors

ISSN

1424-8220

Publisher

MDPI

Issue

5

Volume

12

Page range

5650-5669

Department affiliated with

  • Informatics Publications

Full text available

  • Yes

Peer reviewed?

  • Yes

Contributors

Jochen Arlt, David Tyndall, Richard Walker, Daniel Matthews, Viput Visitkul, Justin Richardson, R K Henderson

Legacy Posted Date

2012-05-21

First Open Access (FOA) Date

2012-05-21

First Compliant Deposit (FCD) Date

2012-05-02

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