ATR-mediated_phosphorylation_of_DNA_etc.pdf (2.05 MB)
ATR-mediated phosphorylation of DNA polymerase ? is needed for efficient recovery from UV damage
journal contribution
posted on 2023-06-08, 11:03 authored by Thomas Göhler, Simone Sabbioneda, Catherine M Green, Alan LehmannAlan LehmannDNA polymerase ? (pol?) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, pol? becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of pol? depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of pol? but not its PCNA-interacting motif. ATR-dependent phosphorylation of pol? is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.
History
Publication status
- Published
File Version
- Accepted version
Journal
Journal of Cell BiologyISSN
0021-9525Publisher
Rockefeller University PressExternal DOI
Issue
2Volume
192Page range
219-227Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Full text available
- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2012-04-23First Open Access (FOA) Date
2012-04-23First Compliant Deposit (FCD) Date
2012-02-29Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC