ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage

Göhler, Thomas, Sabbioneda, Simone, Green, Catherine M and Lehmann, Alan R (2011) ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage. Journal of Cell Biology, 192 (2). pp. 219-227. ISSN 0021-9525

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Abstract

DNA polymerase η (polη) belongs to the Y-family of DNA polymerases and facilitates translesion synthesis past UV damage. We show that, after UV irradiation, polη becomes phosphorylated at Ser601 by the ataxia-telangiectasia mutated and Rad3-related (ATR) kinase. DNA damage–induced phosphorylation of polη depends on its physical interaction with Rad18 but is independent of PCNA monoubiquitination. It requires the ubiquitin-binding domain of polη but not its PCNA-interacting motif. ATR-dependent phosphorylation of polη is necessary to restore normal survival and postreplication repair after ultraviolet irradiation in xeroderma pigmentosum variant fibroblasts, and is involved in the checkpoint response to UV damage. Taken together, our results provide evidence for a link between DNA damage–induced checkpoint activation and translesion synthesis in mammalian cells.

Item Type: Article
Keywords: UV damage, phosphorylation, DNA polymerase
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Philippa Erasmus
Date Deposited: 23 Apr 2012 11:14
Last Modified: 14 Mar 2017 00:37
URI: http://sro.sussex.ac.uk/id/eprint/38210

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