Isolation and characterization of Ty1-copia retrotransposon sequences in the blue agave (Agave tequilana Weber var. azul) and their development as SSAP markers for phylogenetic analysis

Bousios, Alexandros, Saldana-Oyarzabal, Ivan, Valenzuela-Zapata, Ana G, Wood, Carlton and Pearce, Stephen R (2007) Isolation and characterization of Ty1-copia retrotransposon sequences in the blue agave (Agave tequilana Weber var. azul) and their development as SSAP markers for phylogenetic analysis. Plant Science, 172 (2). pp. 291-298. ISSN 0168-9452

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Abstract

Agave tequilana Weber variety ``azul is the only variety of blue agave which is accepted for the production of tequila, but the phylogenetic relationships between this variety and other agave germplasm is still poorly understood. Retrotransposon-based molecular markers have proven to be highly informative in determining phylogenetic relationships in other crop systems. We report the isolation of a population of Ty1-copia retrotransposon terminal sequences from A. tequilana and develop three of these as sequence-specific amplification polymorphism (SSAP) molecular genetic markers. SSAP analysis shows high levels of retrotransposon polymorphism throughout agave varieties and species and identifies the tequila agaves as a distinct phylogenetic group. The retrotransposon SSAP markers presented here represent a powerful new tool for germplasm assessment in the agaves.

Item Type: Article
Additional Information: The low genetic diversity of tequila germplasm is a major problem for industry. This work describes the first development of high resolution molecular markers in agave species and represents the most informative technology to date in assessing tequila germplasm which has the potential to improve sustainability in the tequila industry.
Depositing User: Alexandros Bousios
Date Deposited: 21 Feb 2012 10:00
Last Modified: 30 Nov 2012 17:11
URI: http://sro.sussex.ac.uk/id/eprint/37551
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