Genetic diversity of SIRE-1 retroelements in annual and perrenial glycine species revealed using SSAP

Chesnay, Catherine, Kumar, Amar and Pearce, Stephen R (2007) Genetic diversity of SIRE-1 retroelements in annual and perrenial glycine species revealed using SSAP. Cellular and Molecular Biology Letters, 12 (1). pp. 103-110. ISSN 1425-8153

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Abstract

Sequence Specific Amplification Polymorphisms (SSAP) were used to measure the distribution and structure of SIRE-1 retroelement populations in annual and perennial Glycine species. For SSAP analysis, primers corresponding to a region immediately upstream of the 3' LTR of the soybean retroelement SIRE-1 were chosen. Analysis reveals that SIRE-1 is present throughtout the Glycine genus and shows that the annual species have similar SIRE-1 populations whilst the perennial species have much more distinct and diverse populations. The high number of species-specific subgroups suggest that SIRE-1 has been active and evolving independently in each species during the course of Glycine evolution.

Item Type: Article
Additional Information: This publication uses the insertional polymorphism of one of the very few putative plant retroviruses (SIRE-1) to assess germplasm diversity in the commercially important Glycine genus. The data demonstrates that SIRE-1 is ancient and widespread which overturns the conventional view that SIRE-1 is a recent acquisition to the soybean genome.
Depositing User: Stephen Pearce
Date Deposited: 21 Feb 2012 10:00
Last Modified: 13 Apr 2012 12:09
URI: http://sro.sussex.ac.uk/id/eprint/37489
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