Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR

Tam, Sheh May, Mhiri, Corinne, Vogelaar, Aat, Kerkveld, Marcel, Pearce, Steve R and Grandbastien, Marie-Angele (2007) Comparative analyses of genetic diversities within tomato and pepper collections detected by retrotransposon-based SSAP, AFLP and SSR. TAG Theoretical and Applied Genetics, 110 (5). pp. 819-831. ISSN 0040-5752

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Abstract

The retrotransposon-based sequence-specific amplification polymorphism (SSAP) marker system was used to assess the genetic diversities of collections of tomato and pepper industrial lines. The utility of SSAP markers was compared to that of amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. On the basis of our results, SSAP is most informative of the three systems for studying genetic diversity in tomato and pepper, with a significant correlation of genetic relationships between different SSAP datasets and between SSAP, AFLP and SSR markers. SSAP showed about four- to ninefold more diversity than AFLP and had the highest number of polymorphic bands per assay ratio and the highest marker index. For tomato, SSAP is more suitable for inferring overall genetic variation and relationships, while SSR has the ability to detect specific genetic relationships. All three marker results for pepper showed general agreement with pepper types. Additionally, retrotransposon sequences isolated from one species can be used in related Solanaceae genera. These results suggest that different marker systems are suited for studying genetic diversity in different contexts depending on the group studied, where discordance between different marker systems can be very informative for understanding genetic relationships within the study group.

Item Type: Article
Additional Information: This publication demonstrates for the first time the high value of retrotansposon-based markers by directly comparing them to other more commonly used systems such as SSR and AFLP. SP conceived the study and carried out the retrotransposon marker isolation, characterisation and analysis, comprising a substantial contribution to the work.
Depositing User: Stephen Pearce
Date Deposited: 21 Feb 2012 10:00
Last Modified: 25 Jun 2012 13:32
URI: http://sro.sussex.ac.uk/id/eprint/37466
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