Protein-RNA interactions: a structural analysis

Jones, Susan, Daley, David T A, Luscombe, Nicholas M, Berman, Helen M and Thornton, Janet M (2001) Protein-RNA interactions: a structural analysis. Nucleic Acids Research, 29 (4). pp. 943-954. ISSN 0305-1048

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Abstract

A detailed computational analysis of 32 protein¿RNA complexes is presented. A number of physical and chemical properties of the intermolecular interfaces are calculated and compared with those observed in protein¿double-stranded DNA and protein¿single-stranded DNA complexes. The interface properties of the protein¿RNA complexes reveal the diverse nature of the binding sites. van der Waals contacts played a more prevalent role than hydrogen bond contacts, and preferential binding to guanine and uracil was observed. The positively charged residue, arginine, and the single aromatic residues, phenylalanine and tyrosine, all played key roles in the RNA binding sites. A comparison between protein¿RNA and protein¿DNA complexes showed that whilst base and backbone contacts (both hydrogen bonding and van der Waals) were observed with equal frequency in the protein¿RNA complexes, backbone contacts were more dominant in the protein¿DNA complexes. Although similar modes of secondary structure interactions have been observed in RNA and DNA binding proteins, the current analysis emphasises the differences that exist between the two types of nucleic acid binding protein at the atomic contact level.

Item Type: Article
Additional Information: SJ wrote the algorithms, conducted the analysis and was the corresponding author
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Sue Jones
Date Deposited: 06 Feb 2012 21:29
Last Modified: 06 Mar 2017 08:32
URI: http://sro.sussex.ac.uk/id/eprint/31467

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