Cloning and expression of a leech complexin

Dykes, Iain M and Davies, Jane A (2004) Cloning and expression of a leech complexin. Mechanisms of Development, 4 (1). pp. 93-97. ISSN 0925-4773

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Abstract

Release of neurotransmitter from presynaptic nerve terminals is mediated by SNARE proteins, which are located on the vesicle and plasma membranes. These proteins form a SNARE complex thought to mediate membrane fusion. Complexin is a soluble protein essential for transmitter release, which has been postulated to bind to and stabilise the SNARE complex. We have cloned a complexin homologue, Hm-cpx1, from the leech, Hirudo medicinalis. This protein is expressed in only a subset of neurons in the leech CNS, including the Retzius and P neurons. It is 33% identical to rat complexin I, and 44% identical to squid complexin. Sequence conservation is particularly high in the predicted SNARE binding domain.

Item Type: Article
Additional Information: We examined complexin expression in several identified neurones in Hirudo medicinalis, confirming the basic mechanisms of neurotransmitter release are similar to vertebrates. This now raises the exciting possibility of manipulating transmission at very well characterised specific synapses. Dykes was Davies' DPhil student.
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Depositing User: Jane Davies
Date Deposited: 06 Feb 2012 21:27
Last Modified: 22 Mar 2012 12:17
URI: http://sro.sussex.ac.uk/id/eprint/31313
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