End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae

Neale, Matt and Keeney, S (2009) End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae. Methods in Molecular Biology, 557. pp. 183-195. ISSN 10643745

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Abstract

During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process. Spo11 is removed from the DSB by single-stranded endonucleolytic cleavage flanking the DSB, liberating a short-lived species consisting of Spo11 protein covalently linked to a short oligonucleotide. The method presented here details how to detect these Spo11-oligo complexes in extracts made from meiotic yeast cells.

Item Type: Article
Additional Information: Source Type: Book series (ISBN: 978-193411566-4), Document Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
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Depositing User: Matt Neale
Date Deposited: 06 Feb 2012 21:22
Last Modified: 30 Jul 2012 08:38
URI: http://sro.sussex.ac.uk/id/eprint/30985
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