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Compelling EPR evidence that the alternative oxidase is a diiron carboxylate protein
journal contribution
posted on 2023-06-08, 09:37 authored by Anthony Moore, Jane E Carré, Charles Affourtit, Mary Albury, Paul G Crichton, Kiyoshi Kita, Peter HeathcoteThe alternative oxidase is a respiratory chain protein found in plants, fungi and some parasites that still remains physically uncharacterised. In this report we present EPR evidence from parallel mode experiments which reveal signals at approximately g = 16 in both purified alternative oxidase protein (g = 16.9), isolated mitochondrial membranes (g = 16.1), and in trypanosomal AOX expressed in Escherichia coli membranes (g = 16.4). Such signals are indicative of a dicarboxylate diiron centre at the active site of the enzyme. To our knowledge these data represent the first EPR signals from AOX present in its native environment.
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Publication status
- Published
Journal
BBA - Biochimica et Biophysica ActaISSN
0006-3002Publisher
ElsevierExternal DOI
Volume
1777Page range
327-330Pages
4.0Department affiliated with
- Biochemistry Publications
Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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