Control of Drosophila imaginal disc development by rotund and roughened eye: differentially expressed transcripts of the same gene encoding distinct zinc finger proteins

St Pierre, Susan E, Galindo-, Maximo I, Couso, Juan P and Thor, Stefan (2002) Control of Drosophila imaginal disc development by rotund and roughened eye: differentially expressed transcripts of the same gene encoding distinct zinc finger proteins. Development, 129 (5). pp. 1273-1281. ISSN 0950-1991

Full text not available from this repository.

Abstract

The Drosophila rotund gene is required in the wings, antenna, haltere, proboscis and legs. A member of the Rac family of GTPases, denoted the rotund racGAP gene, was previously identified in the rotund region. However, previous studies indicated that rotund racGAP was not responsible for the rotund phenotypes and that the rotund gene had yet to be identified. We have isolated the rotund gene and show that it is a member of the Krppel family of zinc finger genes. The adjacent roughened eye locus specifically affects the eye and is genetically separable from rotund. However, roughened eye and rotund are tightly linked, and we have therefore also isolated the roughened eye transcript. Intriguingly, we show that roughened eye is part of the rotund gene but is represented by a different transcript. The rotund and roughened eye transcripts result from the utilization of two different promoters that direct expression in non-overlapping domains in the larval imaginal discs. The predicted Rotund and Roughened Eye proteins share the same C-terminal region, including the zinc finger domain, but differ in their N-terminal regions. Each cDNA can rescue only the corresponding mutation and show negative effects when expressed in each others domain of expression. These results indicate that in addition to the differential expression of rotund and roughened eye, their proteins have distinct activities. rotund and roughened eye act downstream of early patterning genes such as dachshund and appear to be involved in Notch signaling by regulating Delta, scabrous and Serrate.

Item Type: Article
Additional Information: Myself and postdoc Ibo Galindo contributed. The Drosophila Genome Project had missed this locus. We showed that it encodes two transcripts with small sequence differences but opposite activities confined to imaginal development. Thus, the strategy of the Genome Project to identify genes -computational searches of embryonic cDNA libraries- was incomplete.
Schools and Departments: School of Life Sciences > Biochemistry
Brighton and Sussex Medical School > Clinical and Experimental Medicine
Depositing User: Maximo Ibo Galindo-Orozco
Date Deposited: 06 Feb 2012 21:12
Last Modified: 21 Sep 2017 10:43
URI: http://sro.sussex.ac.uk/id/eprint/30158
📧 Request an update