Temperature-dependent modulation of interference and chromosome segregation in msh4 mutants of budding yeast

Chan, Andrew Chi-ho, Borts, Rhona H and Hoffmann, Eva (2009) Temperature-dependent modulation of interference and chromosome segregation in msh4 mutants of budding yeast. PLoS ONE, 4. e7284. ISSN 1932-6203

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Abstract

BACKGROUND: In many organisms, homologous chromosomes rely upon recombination-mediated linkages, termed crossovers, to promote their accurate segregation at meiosis I. In budding yeast, the evolutionarily conserved mismatch-repair paralogues, Msh4 and Msh5, promote crossover formation in conjunction with several other proteins, collectively termed the Synapsis Initiation Complex (SIC) proteins or 'ZMM's (Zip1-Zip2-Zip3-Zip4-Spo16, Msh4-Msh5, Mer3). zmm mutants show decreased levels of crossovers and increased chromosome missegregation, which is thought to cause decreased spore viability. PRINCIPAL FINDINGS: In contrast to other ZMM mutants, msh4 and msh5 mutants show improved spore viability and chromosome segregation in response to elevated temperature (23 degrees C versus 33 degrees C). Crossover frequencies in the population of viable spores in msh4 and msh5 mutants are similar at both temperatures, suggesting that temperature-mediated chromosome segregation does not occur by increasing crossover frequencies. Furthermore, meiotic progression defects at elevated temperature do not select for a subpopulation of cells with improved segregation. Instead, another ZMM protein, Zip1, is important for the temperature-dependent improvement in spore viability. CONCLUSIONS: Our data demonstrate interactions between genetic (zmm status) and environmental factors in determining chromosome segregation.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Eva Hoffmann
Date Deposited: 06 Feb 2012 21:10
Last Modified: 07 Mar 2017 08:58
URI: http://sro.sussex.ac.uk/id/eprint/29945

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