Interference of papillomavirus E6 protein with single-strand break repair by interaction with XRCC1

Iftner, Thomas, Elbel, Michaela, Schopp, Betti, Hiller, Thomas, Loizou, Joanna I, Caldecott, Keith W and Stubenrauch, Frank (2002) Interference of papillomavirus E6 protein with single-strand break repair by interaction with XRCC1. EMBO Journal, 21 (17). pp. 4741-4748. ISSN 0261-4189

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Abstract

XRCC1 protein is required for the repair of DNA single-strand breaks and genetic stability, and is essential for viability in mammals. XRCC1 functions as a scaffold protein by interacting and modulating polypeptide components of the single-strand break repair machinery, including AP endonuclease-1, DNA ligase III, poly (ADP-ribose) polymerase, DNA polymerase and human polynucleotide kinase. We show here that the E6 protein of human papillomavirus type 1, 8 and 16 directly binds XRCC1. When tested in CHO derived XRCC1 'knock out' EM9 cells, co-expression of human papillomavirus 16 E6 with human XRCC1 reduced the ability of the latter protein to correct the methyl methane sulfate sensitivity of XRCC1 mutant CHO cell line EM9. These data identify a novel link between small DNA tumour viruses and DNA repair pathways, and suggest a novel explanation for the development of genomic instability in tissue cells persistently infected with papillomaviruses.

Item Type: Article
Additional Information: From SRO Other External: T. Iftner, F. Stubenrauch
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Keith Caldecott
Date Deposited: 06 Feb 2012 21:07
Last Modified: 14 Jun 2012 10:47
URI: http://sro.sussex.ac.uk/id/eprint/29667
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