Analysis of genes from inner ear developmental-stage cDNA subtraction reveals molecular regionalisation of the otic capsule

Ficker, Michael, Powles, Nicola, Warr, Nick, Pirvola, Ulla and Maconochie, Mark (2004) Analysis of genes from inner ear developmental-stage cDNA subtraction reveals molecular regionalisation of the otic capsule. Developmental Biology, 268 (1). pp. 7-23. ISSN 0012-1606

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Abstract

Although the gross embryology of inner ear development has been documented for several different vertebrate species at a descriptive level, our understanding of the molecular mechanisms involved remains rudimentary. Therefore, we have used cDNA subtraction and normalization procedures to define genes upregulated in the 13.5dpc mouse inner ear, a developmental stage where inner ear morphogenesis and tissue remodeling is active and differentiation of future hair cells is being initiated. We recovered 33 different genes from this subtraction and using gene-specific primers have confirmed the transcriptional upregulation of 26 of these in the 13.5dpc inner ear. Northern analyses were used to investigate splicing differences between the inner ear and the whole embryo at 13.5dpc. Spatial localization of expression was determined through whole-ear in situ hybridization analysis, and selected genes were analyzed in more detail through in situ hybridization of tissue sections. These data illustrate that the genes isolated in this study are expressed in the developing otic capsule and/or neuroepithelium. Furthermore, the expression patterns also reveal molecular heterogeneity in the developing capsule and indicate that for some genes, the chondrogenic otic capsule is composed of distinct domains of gene expression.

Item Type: Article
Additional Information: I am corresponding author, on work almost exclusively coming from my lab (1 collaborator UP, other authors my lab)
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: Mark Maconochie
Date Deposited: 06 Feb 2012 21:07
Last Modified: 26 Mar 2012 13:44
URI: http://sro.sussex.ac.uk/id/eprint/29576
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