DNA polymerase η is an A-T mutator in somatic hypermutation of immunoglobulin variable genes

Zeng, Xianmin, Winter, David B, Kasmer, Cynthia, Kraemer, Kenneth H, Lehmann, Alan R and Gearhart, Patricia J (2001) DNA polymerase η is an A-T mutator in somatic hypermutation of immunoglobulin variable genes. Nature Immunology, 2. pp. 537-541. ISSN 1529-2908

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Abstract

To determine whether DNA polymerase plays a role in the hypermutation of immunoglobulin variable genes, we examined the frequency and pattern of substitutions in variable VH6 genes from the peripheral blood lymphocytes of three patients with xeroderma pigmentosum variant disease, whose polymerase had genetic defects. The frequency of mutation was normal but the types of base changes were different: there was a decrease in mutations at A and T and a concomitant rise in mutations at G and C. We propose that more than one polymerase contributes to hypermutation and that if one is absent, others compensate. The data indicate that polymerase is involved in generating errors that occur predominantly at A and T and that another polymerase(s) may preferentially generate errors opposite G and C.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Alan Lehmann
Date Deposited: 06 Feb 2012 21:05
Last Modified: 03 Apr 2012 09:19
URI: http://sro.sussex.ac.uk/id/eprint/29430
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