19F Nuclear Magnetic Resonance Chemical Shifts of Flourine Containing Aliphatic Amino Acids in Proteins: Studies on Lactobacillus casei DihydrofolateReductase Containing (2S 4S )-5-Flouroleucine II

Feeney, James, McCormick, John E, Bauer, Christopher J, Birdsall, Berry, Moody, Claire M, Starkmann, Bernard A, Young, Douglas W, Francis, Peter, Havlin, Robert H, Arnold, William D and Oldfield, Eric (1996) 19F Nuclear Magnetic Resonance Chemical Shifts of Flourine Containing Aliphatic Amino Acids in Proteins: Studies on Lactobacillus casei DihydrofolateReductase Containing (2S 4S )-5-Flouroleucine II. Journal of the American Chemical Society, 118. 8700 - 8706. ISSN 0002-7863

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Abstract

We have prepared Lactobacillus casei dihydrofolate reductase containing biosynthetically incorporated (2S,4S)-5-fluoroleucine ([5-F]-Leu DHFR) and have obtained its 1H and 19F NMR spectra at 9.4 Tesla. The 19F spectrum of [5-F]-Leu DHFR showed 12 fairly sharp peaks (one containing two overlapped signals) for the 13 leucine residues in DHFR, covering a chemical shift range of 15 ppm. The large range of chemical shifts observed could not be explained solely in terms of the electrostatic field effects due to local charge fields and is thought to have a second contribution from side-chain conformational differences (γ-gauche effects) between different leucine residues, making 19F NMR of aliphatic amino acids in proteins a potentially useful new probe of protein structure.

Item Type: Article
Schools and Departments: School of Life Sciences > Chemistry
Depositing User: EPrints Services
Date Deposited: 06 Feb 2012 21:02
Last Modified: 24 Jul 2013 14:30
URI: http://sro.sussex.ac.uk/id/eprint/29210
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