The Gam protein of phage Mu is an orthologue of eukaryotic Ku.

d'Adda di Fagagna, Fabrizio, Weller, Geoffrey R, Doherty, Aidan J and Jackson, Stephen P (2003) The Gam protein of phage Mu is an orthologue of eukaryotic Ku. EMBO Reports, 4 (1). pp. 47-52. ISSN 1469-221X

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Abstract

Mu bacteriophage inserts its DNA into the genome of host bacteria and is used as a model for DNA transposition events in other systems. The eukaryotic Ku protein has key roles in DNA repair and in certain transposition events. Here we show that the Gam protein of phage Mu is conserved in bacteria, has sequence homology with both subunits of Ku, and has the potential to adopt a similar architecture to the core DNA-binding region of Ku. Through biochemical studies, we demonstrate that Gam and the related protein of Haemophilus influenzae display DNA binding characteristics remarkably similar to those of human Ku. In addition, we show that Gam can interfere with Ty1 retrotransposition in Saccharomyces cerevisiae. These data reveal structural and functional parallels between bacteriophage Gam and eukaryotic Ku and suggest that their functions have been evolutionarily conserved.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Aidan Doherty
Date Deposited: 06 Feb 2012 20:49
Last Modified: 03 Apr 2012 07:41
URI: http://sro.sussex.ac.uk/id/eprint/28349
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