Construction and application of streptomycete promoter probe vectors which employ the Streptomyces glaucescens tyrosinase-encoding gene as reporter

Paget, Mark S B, Hintermann, Gilberto and Smith, Colin P (1994) Construction and application of streptomycete promoter probe vectors which employ the Streptomyces glaucescens tyrosinase-encoding gene as reporter. Gene, 146 (1). pp. 105-110. ISSN 03781119

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Abstract

We have constructed multicopy and integrative streptomycete promoter probe vectors that employ the promoterless tyrosinase (melC) operon of Streptomyces glaucescens as the chromogenic transcriptional reporter. Each vector contains the reporter cassette, RC3, which comprises part of the melC operon flanked by transcription terminators; RC3 may be easily inserted into any vector. We demonstrate the use of the pIJ101-based mel vector, pMT3010, for the isolation of mutations which alter expression of the S. coelicolor glycerol operon. The S. glaucescens mel reporter system is well-suited for the visual, non-selective identification of regulatory mutants and can be used efficiently for screening several thousand clones at high colony density.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Mark Paget
Date Deposited: 06 Feb 2012 20:45
Last Modified: 22 Mar 2012 15:11
URI: http://sro.sussex.ac.uk/id/eprint/27948
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