Extrasynaptic vesicle recycling in mature hippocampal neurons

Ratnayaka, Arjuna, Marra, Vincenzo, Branco, Tiago and Staras, Kevin (2011) Extrasynaptic vesicle recycling in mature hippocampal neurons. Nature Communications, 2 (11). 531a. ISSN 2041-1723

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Abstract

Fast neuronal signalling relies on highly-regulated vesicle fusion and recycling at specialized presynaptic terminals. Recently, examples of non-classical neurotransmission have also been reported, where fusion of vesicles can occur at sites remote from conventional synapses. This has potentially broad biological implications, but the underlying mechanisms are not well established. Here, in mature hippocampal neurons, we demonstrate that a complete vesicle recycling pathway can occur at discrete axonal sites and that extrasynaptic fusion is a robust feature of native tissue. We show that laterally mobile vesicle clusters trafficking between synaptic terminals become transiently stabilized by evoked action potentials and undergo complete but delayed Ca2+-dependent fusion along axons. This fusion is associated with dynamic actin accumulation and subsequently, vesicles can be locally recycled, reacidified and re-used. Immunofluorescence and ultrastructural work demonstrates that extrasynaptic fusion sites can have apposed postsynaptic specializations, suggesting that mobile vesicle recycling may underlie highly dynamic neuron-neuron communication.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Subjects: Q Science
Depositing User: Kevin Staras
Date Deposited: 06 Feb 2012 20:43
Last Modified: 03 Sep 2013 14:49
URI: http://sro.sussex.ac.uk/id/eprint/27721
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