Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp israelensis in Asticcacaulis excentricus

Zheng, Dasheng, Valdez-Cruz, Norma Adriana, Armengol, Gemma, Sevrez, Chloe, Munoz-Olaya, Jose Maurilio, Yuan, Zhiming, Orduz, Sergio and Crickmore, Neil (2007) Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp israelensis in Asticcacaulis excentricus. Current Microbiology, 54 (1). pp. 58-62. ISSN 0343-8651

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Abstract

The cyt1Aa gene from Bacillus thuringiensis subsp. israelensis (Bti), whose product synergizes other mosquitocidal toxins, and functions as a repressor of resistance developed by mosquitoes against Bacilli insecticides, was introduced into the aquatic Gram-negative bacterium Asticcacaulis excentricus alongside the cry11Aa gene. The genes were introduced as an operon, but although mRNA was detected for both genes, no Cyt1Aa toxin was detected. Both proteins were expressed using a construct in which a promoter was inserted upstream of each gene. Recombinant A. excentricus expressing both toxins was found to be approximately twice as toxic to third instar larvae of Culex quinquefasciatus as transformants expressing just Cry11Aa.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: EPrints Services
Date Deposited: 06 Feb 2012 20:41
Last Modified: 22 Mar 2012 14:50
URI: http://sro.sussex.ac.uk/id/eprint/27452
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