Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling

Powles, Nicola, Marshall, Heather, Economou, Androulla, Chiang, Chin, Murakami, Akira, Dickson, Clive, Krumlauf, Robb and Maconochie, Mark (2004) Regulatory analysis of the mouse Fgf3 gene: control of embryonic expression patterns and dependence upon sonic hedgehog (Shh) signalling. Developmental Dynamics, 230 (1). pp. 44-56. ISSN 1058-8388

Full text not available from this repository.

Abstract

Fgf3 displays a dynamic and complex expression pattern during mouse embryogenesis. To address the molecular mechanisms underlying Fgf3 expression, we used a transgenic approach to assay genomic regions from the mouse Fgf3 gene for regulatory activity. We identified an enhancer that mediates major components of embryonic expression, governing expression in the midbrain, hindbrain, surface ectoderm, dorsal roots and dorsal root ganglia (DRG), proximal sensory ganglia, and the developing central nervous system (CNS). Deletional analysis of the enhancer further delimited this regulatory activity to a 5.7-kb fragment. We have also revealed sonic hedgehog (Shh) -dependent and Shh-independent aspects of Fgf3 expression through breeding the Fgf3 reporter transgene into Shh mutants. In the absence of Shh signalling, Fgf3 reporter expression is lost in the ventral CNS, DRG, and superior cervical nerves, whereas activation of reporter expression in cranial ganglion cells is Shh independent. Moreover, detailed re-examination of the Shh phenotype revealed that Shh signalling is required for the correct development/maturation of the DRG. Developmental Dynamics 230:44¿56, 2004. © 2004 Wiley-Liss, Inc.

Item Type: Article
Schools and Departments: School of Life Sciences > Neuroscience
Depositing User: EPrints Services
Date Deposited: 06 Feb 2012 20:39
Last Modified: 26 Mar 2012 12:15
URI: http://sro.sussex.ac.uk/id/eprint/27299
📧 Request an update