Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus

Armengol, Gemma, Guevara, Oscar Enrique, Orduz, Sergio and Crickmore, Neil (2005) Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus. Current Microbiology, 51 (6). pp. 430-433. ISSN 0343-8651

Full text not available from this repository.

Abstract

A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC50 of 6.83 x 10(5) cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Neil Crickmore
Date Deposited: 06 Feb 2012 20:39
Last Modified: 12 Jun 2012 13:46
URI: http://sro.sussex.ac.uk/id/eprint/27296
📧 Request an update