Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli

Baba-Dikwa, Aisha, Thompson, Darren, Spencer, Nick J, Andrews, Simon C and Watson, Kimberly A (2008) Overproduction, purification and preliminary X-ray diffraction analysis of YncE, an iron-regulated Sec-dependent periplasmic protein from Escherichia coli. Acta Crystallographica Section F, 64 (10). pp. 966-969. ISSN 1744-3091

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Abstract

The yncE gene of Escherichia coli encodes a predicted periplasmic protein of unknown function. The gene is de-repressed under iron restriction through the action of the global iron regulator Fur. This suggests a role in iron acquisition, which is supported by the presence of the adjacent yncD gene encoding a potential TonB-dependent outer-membrane transporter. Here, the preliminary crystallographic structure of YncE is reported, revealing that it consists of a seven-bladed [beta]-propeller which resembles the corresponding domain of the `surface-layer protein' of Methanosarcina mazei. A full structure determination is under way in order to provide insight into the function of this protein.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Darren Thompson
Date Deposited: 06 Feb 2012 20:37
Last Modified: 07 Jun 2012 11:06
URI: http://sro.sussex.ac.uk/id/eprint/27064
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