Mol._Cell._Biol.-1994-Murray-4878-88.pdf (2.44 MB)
Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage
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posted on 2023-06-08, 06:21 authored by Jo Murray, M Tavassoli, R Al-Harithy, K S Sheldrick, Alan LehmannAlan Lehmann, Antony CarrAntony Carr, Felicity WattsThe rad2 mutant of Schizosaccharomyces pombe is sensitive to UV irradiation and deficient in the repair of UV damage. In addition, it has a very high degree of chromosome loss and/or nondisjunction. We have cloned the rad2 gene and have shown it to be a member of the Saccharomyces cerevisiae RAD2/S. pombe rad13/human XPG family. Using degenerate PCR, we have cloned the human homolog of the rad2 gene. Human cDNA has 55% amino acid sequence identity to the rad2 gene and is able to complement the UV sensitivity of the rad2 null mutant. We have thus isolated a novel human gene which is likely to be involved both in controlling the fidelity of chromosome segregation and in the repair of UV-induced DNA damage. Its involvement in two fundamental processes for maintaining chromosomal integrity suggests that it is likely to be an important component of cancer avoidance mechanisms.
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Publication status
- Published
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- Published version
Journal
Molecular and Cellular BiologyISSN
0270-7306Publisher
American Society for MicrobiologyIssue
7Volume
14Page range
4878-4888Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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- Yes
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06First Open Access (FOA) Date
2016-03-22First Compliant Deposit (FCD) Date
2016-11-16Usage metrics
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