Cloning of the Retinoblastoma cDNA from the Japanese medaka (Oryzias latipes) and preliminary evidence of mutational alterations in chemically-induced retinoblastomas

Rotchell, J M, Blair, J B, Shim, J-K, Hawkins, W E and Ostrander, G K (2001) Cloning of the Retinoblastoma cDNA from the Japanese medaka (Oryzias latipes) and preliminary evidence of mutational alterations in chemically-induced retinoblastomas. Gene, 263 (1-2). pp. 231-237. ISSN 0378-1119

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Abstract

We have cloned a medaka homolog of the human retinoblastoma (Rb) susceptibility gene. The medaka Rb cDNA encodes a predicted protein of 909 amino acids. DNA sequence analysis with other vertebrate Rb sequences demonstrates that the medaka Rb cDNA is highly conserved in regions of functional importance. An antibody raised against an epitope of the human pRb recognizes the protein product of the medaka Rb gene, detecting a 105 kDa protein in all tissues examined and at differential levels for the stages of embryonic development studied. The sequence reported herein, combined with the high degree of conservation observed in critical domains, has also facilitated a preliminary investigation of the molecular etiology of chemically-induced retinoblastoma. The mutational alterations characterized suggest that medaka may provide a novel model and, thus, provide additional insight into the human retinoblastoma condition.

Item Type: Article
Additional Information: This is a novel interdisciplinary result, describing the development of a fish model for human retinoblastoma. I was funded by NIH (as a postdoc at Johns Hopkins University) and BBSRC (as principal investigator), and is recognition that fish can be a suitable surrogate for studying certain cancers.
Schools and Departments: School of Life Sciences > Biology and Environmental Science
Depositing User: Jeanette Rotchell
Date Deposited: 06 Feb 2012 20:22
Last Modified: 30 Nov 2012 17:06
URI: http://sro.sussex.ac.uk/id/eprint/25648
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