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Suppression of homologous recombination by the Saccharomyces cerevisiae linker histone.
journal contribution
posted on 2023-06-08, 06:03 authored by Jessica A Downs, Effie Kosmidou, Alan Morgan, Stephen P JacksonThe basic unit of chromatin in eukaryotes is the nucleosome, comprising 146 bp of DNA wound around two copies of each of four core histones. Chromatin is further condensed by association with linker histones. Saccharomyces cerevisiae Hho1p has sequence homology to other known linker histones and interacts with nucleosomes in vitro. However, disruption of HHO1 results in no significant changes in the phenotypes examined thus far. Here, we show that Hho1p is inhibitory to DNA repair by homologous recombination (HR). We find Hho1p is abundant and associated with the genome, consistent with a global role in DNA repair. Furthermore, we establish that Hho1p is required for a full life span and propose that this is mechanistically linked to its role in HR. Finally, we show that Hho1p is inhibitory to the recombination-dependent mechanism of telomere maintenance. The role of linker histones in genome stability, aging, and tumorigenesis is discussed.
History
Publication status
- Published
Journal
Molecular CellISSN
1097-2765External DOI
Issue
6Volume
11Page range
1685-1692Pages
8.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
The majority of the work in this project, including the initiation, design, generation of data, interpretation of results, and preparation of the manuscript was done by JD. There is one figure in the manuscript which was supplied by the collaborating Morgan lab.Full text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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