Suppression of homologous recombination by the Saccharomyces cerevisiae linker histone.

Downs, Jessica A, Kosmidou, Effie, Morgan, Alan and Jackson, Stephen P (2003) Suppression of homologous recombination by the Saccharomyces cerevisiae linker histone. Molecular Cell, 11 (6). pp. 1685-1692. ISSN 1097-2765

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Abstract

The basic unit of chromatin in eukaryotes is the nucleosome, comprising 146 bp of DNA wound around two copies of each of four core histones. Chromatin is further condensed by association with linker histones. Saccharomyces cerevisiae Hho1p has sequence homology to other known linker histones and interacts with nucleosomes in vitro. However, disruption of HHO1 results in no significant changes in the phenotypes examined thus far. Here, we show that Hho1p is inhibitory to DNA repair by homologous recombination (HR). We find Hho1p is abundant and associated with the genome, consistent with a global role in DNA repair. Furthermore, we establish that Hho1p is required for a full life span and propose that this is mechanistically linked to its role in HR. Finally, we show that Hho1p is inhibitory to the recombination-dependent mechanism of telomere maintenance. The role of linker histones in genome stability, aging, and tumorigenesis is discussed.

Item Type: Article
Additional Information: The majority of the work in this project, including the initiation, design, generation of data, interpretation of results, and preparation of the manuscript was done by JD. There is one figure in the manuscript which was supplied by the collaborating Morgan lab.
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Jessica Downs
Date Deposited: 06 Feb 2012 20:21
Last Modified: 02 Apr 2012 09:03
URI: http://sro.sussex.ac.uk/id/eprint/25591
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