Schizosaccharomyces pombe Cds1(Chk2) regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60

Miyabe, Izumi, Morishita, Takashi, Shinagawa, Hideo and Carr, Antony M (2009) Schizosaccharomyces pombe Cds1(Chk2) regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60. Journal of Cell Science, 122 (20). pp. 3638-3643. ISSN 0021-9533

Full text not available from this repository.

Abstract

The Schizosaccharomyces pombe rad60 gene is essential for cell growth and is involved in repairing DNA double-strand breaks. Rad60 physically interacts with, and is functionally related to, the structural maintenance of chromosomes 5 and 6 protein complex (Smc5/6). Rad60 is phosphorylated in response to hydroxyurea (HU)-induced DNA replication arrest in a Cds1(Chk2)-dependent manner. Rad60 localizes in nucleus in unchallenged cells, but becomes diffused throughout the cell in response to HU. To understand the role of Rad60 phosphorylation, we mutated the putative phosphorylation target motifs of Cds1(Chk2) and have identified two Cds1(Chk2) target residues responsible for Rad60 dispersal in response to HU. We show that the phosphorylation-defective rad60 mutation partially suppresses HU sensitivity and the elevated recombination frequency of smc6-X. Our data suggest that Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Depositing User: Izumi Miyabe
Date Deposited: 06 Feb 2012 20:19
Last Modified: 01 May 2012 14:39
URI: http://sro.sussex.ac.uk/id/eprint/25408
📧 Request an update