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Schizosaccharomyces pombe Cds1(Chk2) regulates homologous recombination at stalled replication forks through the phosphorylation of recombination protein Rad60
journal contribution
posted on 2023-06-08, 05:55 authored by Izumi Miyabe, Takashi Morishita, Hideo Shinagawa, Antony CarrAntony CarrThe Schizosaccharomyces pombe rad60 gene is essential for cell growth and is involved in repairing DNA double-strand breaks. Rad60 physically interacts with, and is functionally related to, the structural maintenance of chromosomes 5 and 6 protein complex (Smc5/6). Rad60 is phosphorylated in response to hydroxyurea (HU)-induced DNA replication arrest in a Cds1(Chk2)-dependent manner. Rad60 localizes in nucleus in unchallenged cells, but becomes diffused throughout the cell in response to HU. To understand the role of Rad60 phosphorylation, we mutated the putative phosphorylation target motifs of Cds1(Chk2) and have identified two Cds1(Chk2) target residues responsible for Rad60 dispersal in response to HU. We show that the phosphorylation-defective rad60 mutation partially suppresses HU sensitivity and the elevated recombination frequency of smc6-X. Our data suggest that Rad60 phosphorylation is required to regulate homologous recombination at stalled replication forks, probably by regulating Smc5/6.
History
Publication status
- Published
Journal
Journal of Cell ScienceISSN
0021-9533Publisher
Company of BiologistsExternal DOI
Issue
20Volume
122Page range
3638-3643Pages
6.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
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- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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