Hed1 regulates Rad51-mediated recombination via a novel mechanism

Busygina, Valeria, Sehorn, Michael G, Shi, Idina Y, Tsubouchi, Hideo, Roeder, G Shirleen and Sung, Patrick (2008) Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes and Development, 22 (6). pp. 786-795. ISSN 0890-9369

Full text not available from this repository.

Abstract

Two RecA orthologs, Rad51 and Dmc1, mediate homologous recombination in meiotic cells. During budding yeast meiosis, Hed1 coordinates the actions of Rad51 and Dmc1 by down-regulating Rad51 activity. It is thought that Hed1-dependent attenuation of Rad51 facilitates formation of crossovers that are necessary for the correct segregation of chromosomes at the first meiotic division. We purified Hed1 in order to elucidate its mechanism of action. Hed1 binds Rad51 with high affinity and specificity. We show that Hed1 does not adversely affect assembly of the Rad51 presynaptic filament, but it specifically prohibits interaction of Rad51 with Rad54, a Swi2/Snf2-like factor that is indispensable for Rad51-mediated recombination. In congruence with the biochemical results, Hed1 prevents the recruitment of Rad54 to a site-specific DNA double-strand break in vivo but has no effect on the recruitment of Rad51. These findings shed light on the function of Hed1 and, importantly, unveil a novel mechanism for the regulation of homologous recombination.

Item Type: Article
Schools and Departments: School of Life Sciences > Sussex Centre for Genome Damage and Stability
Subjects: Q Science
Depositing User: Hideo Tsubouchi
Date Deposited: 06 Feb 2012 20:17
Last Modified: 11 Oct 2012 09:31
URI: http://sro.sussex.ac.uk/id/eprint/25193
📧 Request an update