Optimisation and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissues in transmission electron microscopy.

Thorpe, Julian R and Harvey, Diana M R (1979) Optimisation and investigation of the use of 2,2-dimethoxypropane as a dehydration agent for plant tissues in transmission electron microscopy. Journal of Ultrastructure Research, 68. pp. 186-194.

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Abstract

After obtaining initially inconsistent results using 2,2-dimethoxypropane (DMP) to dehydrate pea root and stem segments for transmission electron microscopy, it was found that consistent total dehydration of 3-mm pea root and 1- and 3-mm pea stem lengths was only obtained after 2 15 min exposure to DMP, i.e., much longer than originally recommended (L. L. Muller and T. J. Jacks, 1975, J. Histochem. Cytochem., 23, 107). Satisfactory dehydration of a variety of plant tissues was achieved after 2 15 min dehydration in DMP, and no differences in cell ultrastructure were observed between ethanol and DMP-dehydrated samples following three different fixation procedures (glutaraldehyde/OsO4, acrolein/OsO4, and KMnO4). The value of DMP in the retention of cell contents during dehydration was assessed in procedures for the localization of (1) rat liver peroxisomal soluble catalase using 3,3-diaminobenzidine and (2) Na+ K+ and Cl by precipitation techniques in Suaeda maritima leaf segments.

Item Type: Article
Schools and Departments: School of Life Sciences > Biochemistry
Depositing User: Julian Thorpe
Date Deposited: 06 Feb 2012 20:16
Last Modified: 22 Mar 2012 11:41
URI: http://sro.sussex.ac.uk/id/eprint/25108
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