University of Sussex
Browse

File(s) not publicly available

Fission yeast Rad50 stimulates sister chromatid recombination and links cohesion with repair

journal contribution
posted on 2023-06-08, 05:35 authored by E Hartsuiker, E Vaessen, Antony CarrAntony Carr, J Kohli
To study the role of Rad50 in the DNA damage response, we cloned and deleted the Schizosaccharo myces pombe RAD50 homologue. The deletion is sensitive to a range of DNA-damaging agents and shows dynamic epistatic interactions with other recombination¿repair genes. We show that Rad50 is necessary for recombinational repair of the DNA lesion at the mating-type locus and that rad50 shows slow DNA replication. We also find that Rad50 is not required for slowing down S phase in response to hydroxy urea or methyl methanesulfonate (MMS) treatment. Interestingly, in rad50 cells, the recombination frequency between two homologous chromosomes is increased at the expense of sister chromatid recombination. We propose that Rad50, an SMC-like protein, promotes the use of the sister chromatid as the template for homologous recombinational repair. In support of this, we found that Rad50 functions in the same pathway for the repair of MMS-induced damage as Rad21, the homologue of the Saccharomyces cerevisiae Scc1 cohesin protein. We speculate that Rad50 interacts with the cohesin complex during S phase to assist repair and possibly re-initiation of replication after replication fork collapse.

History

Publication status

  • Published

Journal

EMBO Journal

ISSN

0261-4189

Publisher

Nature Publishing Group

Issue

23

Volume

20

Page range

6660-6670

Pages

11.0

Department affiliated with

  • Sussex Centre for Genome Damage Stability Publications

Notes

EH is the main contributor to this publication, I conceived and performed all experiments and wrote the manuscript. This publication describes a novel role of the S. pombe Rad50 protein in linking sister chromatid repair with sister chromatid cohesion

Full text available

  • No

Peer reviewed?

  • Yes

Legacy Posted Date

2012-02-06

Usage metrics

    University of Sussex (Publications)

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC