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Fission yeast Rad50 stimulates sister chromatid recombination and links cohesion with repair
journal contribution
posted on 2023-06-08, 05:35 authored by E Hartsuiker, E Vaessen, Antony CarrAntony Carr, J KohliTo study the role of Rad50 in the DNA damage response, we cloned and deleted the Schizosaccharo myces pombe RAD50 homologue. The deletion is sensitive to a range of DNA-damaging agents and shows dynamic epistatic interactions with other recombination¿repair genes. We show that Rad50 is necessary for recombinational repair of the DNA lesion at the mating-type locus and that rad50 shows slow DNA replication. We also find that Rad50 is not required for slowing down S phase in response to hydroxy urea or methyl methanesulfonate (MMS) treatment. Interestingly, in rad50 cells, the recombination frequency between two homologous chromosomes is increased at the expense of sister chromatid recombination. We propose that Rad50, an SMC-like protein, promotes the use of the sister chromatid as the template for homologous recombinational repair. In support of this, we found that Rad50 functions in the same pathway for the repair of MMS-induced damage as Rad21, the homologue of the Saccharomyces cerevisiae Scc1 cohesin protein. We speculate that Rad50 interacts with the cohesin complex during S phase to assist repair and possibly re-initiation of replication after replication fork collapse.
History
Publication status
- Published
Journal
EMBO JournalISSN
0261-4189Publisher
Nature Publishing GroupExternal DOI
Issue
23Volume
20Page range
6660-6670Pages
11.0Department affiliated with
- Sussex Centre for Genome Damage Stability Publications
Notes
EH is the main contributor to this publication, I conceived and performed all experiments and wrote the manuscript. This publication describes a novel role of the S. pombe Rad50 protein in linking sister chromatid repair with sister chromatid cohesionFull text available
- No
Peer reviewed?
- Yes
Legacy Posted Date
2012-02-06Usage metrics
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