Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm

Li, David D U, Tyndall, David, Walker, Richard, Richardson, Justin, Henderson, Robert K, Arlt, Jochen, Stoppa, David and Charbon, Edoardo (2011) Video-rate fluorescence lifetime imaging camera with CMOS single-photon avalanche diode arrays and high-speed imaging algorithm. Journal of Biomedical Optics, 16 (9). 096012. ISSN 1083-3668

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Abstract

A high-speed and hardware-only algorithm using a center of mass method (CMM) has been proposed for single-detector fluorescence lifetime sensing applications [J. Biomedical Opt. 15, 2010]. This algorithm is now implemented on a field programmable gate array (FPGA) to provide fast lifetime estimates from a 3232 low dark count 0.13m CMOS single-photon avalanche diode (SPAD) plus time-to-digital converter (TDC) array. A simple look-up table (LUT) is included to enhance the lifetime resolvability range and photon economics, making it comparable to the commonly used least-square method (LSM) and maximum-likelihood estimation (MLE) based software. To demonstrate its performance, a widefield microscope was adapted to accommodate the SPAD array and image different test samples. Fluorescence lifetime imaging microscopy (FLIM) on fluorescent beads in Rhodamine 6G at a frame rate of 50fps is also shown.

Item Type: Article
Schools and Departments: School of Engineering and Informatics > Engineering and Design
Depositing User: David Day-Uei Li
Date Deposited: 06 Feb 2012 20:13
Last Modified: 08 Mar 2017 08:31
URI: http://sro.sussex.ac.uk/id/eprint/24737

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